Despite the advancement of new molecular biology techniques like ddPCR or RNAseq, quantitative PCR (qPCR) is still a golden standard for the quantification of nucleic acids, with applications in diagnostics and research. The method is based on real-time monitoring of amplification of the target DNA allowing its quantification (see basics). Yet, for the method to be quantitative, it has to be done properly. Read more
How to present the ratio data? Read more
Which are the most probable reasons for a false positive result and how to address the issue?
Researchers often wonder which statistical tests they should use with their qPCR data. Most frequently these questions arise when analysing gene expression data.
- Which single-cell RNA sequencing data analysis tool to choose in 2022?August 17, 2022 - 8:46 am
- Best Electronic Lab Notebook (ELN) Review for Your Lab in 2021May 26, 2021 - 8:00 am
- The Next Generation Of Antivenom Production Holds Many Possibilities For The FutureMarch 17, 2021 - 8:19 am
- Connected Laboratory & the Role of IoT in ScienceFebruary 4, 2020 - 4:02 pm
- The Importance of Better Digital Data Management in Laboratories and The FAIR FutureFebruary 4, 2020 - 3:06 pm