Despite the advancement of new molecular biology techniques like ddPCR or RNAseq, quantitative PCR (qPCR) is still a golden standard for the quantification of nucleic acids, with applications in diagnostics and research. The method is based on real-time monitoring of amplification of the target DNA allowing its quantification (see basics). Yet, for the method to be quantitative, it has to be done properly. Read more
https://www.splice-bio.com/wp-content/uploads/2017/06/DNA1.jpg 960 1920 Splice https://www.splice-bio.com/wp-content/uploads/2020/12/Splice-logo.png Splice2017-06-08 10:09:422021-09-20 10:08:23qPCR Data Analysis Made Easy
https://www.splice-bio.com/wp-content/uploads/2016/02/ddPCR1.jpg 1249 1500 Katarina Kovac https://www.splice-bio.com/wp-content/uploads/2020/12/Splice-logo.png Katarina Kovac2016-02-16 13:36:572021-09-20 10:08:01Webinar: Why Go Digital in PCR
/by Katarina Kovac
Since we live in the digital era, why shouldn’t a popular method like PCR go digital as well? What is digital PCR (dPCR) and how it is different in comparison to PCR and qPCR, has been explained in the webinar “Why go Digital in qPCR”.
https://www.splice-bio.com/wp-content/uploads/2020/12/Splice-logo.png 0 0 Tea Pavlek https://www.splice-bio.com/wp-content/uploads/2020/12/Splice-logo.png Tea Pavlek2015-06-18 14:00:242021-09-20 10:08:02INTERVIEW: Leading Edge Technologies for Achieving Better Diagnostics
/by Tea Pavlek
https://www.splice-bio.com/wp-content/uploads/2015/05/pc-1207686_1920-1.jpg 1275 1920 Tea Pavlek https://www.splice-bio.com/wp-content/uploads/2020/12/Splice-logo.png Tea Pavlek2015-05-14 12:35:332021-09-20 10:08:02Agents of Change – MIQE Guidelines New iBook
/by Tea Pavlek
Can we trust your published qPCR data?
The pressure to publish is causing shortcuts which result in misleading papers being published.
https://www.splice-bio.com/wp-content/uploads/2015/03/ddd1404BioRad_Droplet-workflow_caption_full-image.jpg 628 1722 Splice https://www.splice-bio.com/wp-content/uploads/2020/12/Splice-logo.png Splice2015-03-10 09:43:502021-09-20 10:08:02Race Towards the Future: Digital Droplet PCR – Conqueror or Ally?
Will ddPCR replace qPCR technology? Read more
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How to present the ratio data? Read more
https://www.splice-bio.com/wp-content/uploads/2015/03/contamination.jpg 785 1300 Splice https://www.splice-bio.com/wp-content/uploads/2020/12/Splice-logo.png Splice2015-03-10 08:58:342021-09-20 10:08:02DNA Contamination of PCR Reagents – a Great Risk for False Positive Signals
Which are the most probable reasons for a false positive result and how to address the issue?
https://www.splice-bio.com/wp-content/uploads/2015/03/Statistic-tests.jpg 905 1280 Splice https://www.splice-bio.com/wp-content/uploads/2020/12/Splice-logo.png Splice2015-03-10 08:44:462021-09-20 10:08:02Parametric or Non-Parametric Tests for qPCR Data?
Researchers often wonder which statistical tests they should use with their qPCR data. Most frequently these questions arise when analysing gene expression data.
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