


Relative Quantification Data Presentation
How to present the ratio data?In gene expression analyses qPCR is most commonly used for relative quantification, meaning that we are comparing one sample to another, treatment to control, one time point to another etc. After we calculate the…

DNA Contamination of PCR Reagents – a Great Risk for False Positive Signals
Which are the most probable reasons for a false positive result and how to address the issue?
If you are using highly conserved 16S rRNA genes (one of the most widely used PCR targets for detection of a broad range of organisms) as a target…

Parametric or Non-Parametric Tests for qPCR Data?
Researchers often wonder which statistical tests they should use with their qPCR data. Most frequently these questions arise when analysing gene expression data.
There are broad groups of statistical tests, parametric and non-parametric.…

“Horsegate” Scandal – PCR Helps You Meet Your Meat Identity Crisis
The scandal has led to the human community in the EU benefiting from the better labeling of foods!
It has been two years since the “Horsegate” scandal broke out when United Kingdom’s Food Standards Agency (FSA) reported findings on…

Recent Attempts to Detect Ebola Virus
Concise overview over the current situation.
Ebolavirus (1) is an enveloped single-stranded RNA virus that has five known species, four of which are pathogenic to humans. The virus was first identified (3) in 1976 in association with…
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