LATEST TRENDS | Splice

INTERVIEW: Think Beyond the PhD – Into the Big World of Small RNAs

Meet Jan Hellemans, CEO of Biogazelle, focused on unraveling the coding and non-coding regions of the genome. "There is more non-coding RNAs in a human cell than protein coding genes. So actually we’ve been ignorant about…

MOLECULAR BIOLOGY AND GENETICS

Excited about Long Reads?

Recently there’s been a lot of excitement building up within the molecular biology community about sequencing long reads, and by “long reads” I mean reads of several kb in length... PacBio has been offering long read sequencing…

MOLECULAR BIOLOGY AND GENETICS

Race Towards the Future: Digital Droplet PCR – Conqueror or Ally?

Will ddPCR replace qPCR technology? A few years ago the efficient technology for splitting samples into hundreds of individual reactions of the same size became available and caused rapid development of the ddPCR. First platforms offered…

MOLECULAR BIOLOGY AND GENETICS, qPCR TIPS

Relative Quantification Data Presentation

How to present the ratio data?In gene expression analyses qPCR is most commonly used for relative quantification, meaning that we are comparing one sample to another, treatment to control, one time point to another etc. After we calculate the…

MOLECULAR BIOLOGY AND GENETICS, qPCR TIPS

DNA Contamination of PCR Reagents – a Great Risk for False Positive Signals

Which are the most probable reasons for a false positive result and how to address the issue? If you are using highly conserved 16S rRNA genes (one of the most widely used PCR targets for detection of a broad range of organisms) as a target…

MOLECULAR BIOLOGY AND GENETICS, qPCR TIPS

Parametric or Non-Parametric Tests for qPCR Data?

Researchers often wonder which statistical tests they should use with their qPCR data. Most frequently these questions arise when analysing gene expression data. There are broad groups of statistical tests, parametric and non-parametric.…

MOLECULAR BIOLOGY AND GENETICS

“Horsegate” Scandal – PCR Helps You Meet Your Meat Identity Crisis

The scandal has led to the human community in the EU benefiting from the better labeling of foods! It has been two years since the “Horsegate” scandal broke out when United Kingdom’s Food Standards Agency (FSA) reported findings on…

MEDICINE

Recent Attempts to Detect Ebola Virus

Concise overview over the current situation. Ebolavirus (1) is an enveloped single-stranded RNA virus that has five known species, four of which are pathogenic to humans. The virus was first identified (3) in 1976 in association with…

COFFEE BREAK NEWS FOR LIFE SCIENTISTS

INTERVIEW: Think Beyond the PhD – Into the Big World of Small RNAs

Excited about Long Reads?

Race Towards the Future: Digital Droplet PCR – Conqueror or Ally?

Relative Quantification Data Presentation

DNA Contamination of PCR Reagents – a Great Risk for False Positive Signals

Parametric or Non-Parametric Tests for qPCR Data?

“Horsegate” Scandal – PCR Helps You Meet Your Meat Identity Crisis

Recent Attempts to Detect Ebola Virus

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COFFEE BREAK NEWS FOR LIFE SCIENTISTS

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